Formulation and Characterization of Myricetin Solid Lipid Nanoparticles (SLNs) via Ultrasonication Method
Main Article Content
Dewi Zulfa Rosida
Muhammad Dzakwan
Hery Muhamad Ansory
Background: Myricetin is a flavonol flavonoid with promising neuroprotective and antioxidant activities. However, its therapeutic application is severely limited by poor water solubility and a low dissolution rate. This study aimed to enhance the solubility and formulation stability of myricetin by developing a Solid Lipid Nanoparticle (SLN) delivery system.
Methods: Myricetin-SLNs were prepared via the ultrasonication method using Tween 80 as a nonionic surfactant. Lipid screening was conducted using Imwitor 491, Dynasan 118, and Apifil at concentrations of 2%, 4%, and 6%. The selected lipid (Apifil at 2%, 3%, 4%, and 5%) was melted at 100 °C, homogenized with the aqueous phase using a magnetic stirrer at 2000 rpm for 1 hour, cooled to 35 °C, and subjected to probe ultrasonication at 35 kHz for 5 minutes. The SLNs were characterized using a particle size analyzer and a UV-Vis spectrophotometer to evaluate storage stability, particle size, polydispersity index (PDI), entrapment efficiency, zeta potential, and DPPH free radical scavenging activity.
Results: Lipid screening revealed that Apifil-based formulations provided superior physical stability compared to Imwitor 491 and Dynasan 118. The optimized formulation, F7 (containing 2% Apifil), yielded the smallest particle size of 105.5±0.70 nm, a highly homogeneous PDI of 0.232, a zeta potential of -20.52 mV, and the highest entrapment efficiency at 73.56%. Furthermore, F7 demonstrated strong antioxidant activity with an IC50 value of 38.77 ppm, compared to pure myricetin (14.44 ppm).
Conclusion: The formulation of myricetin into Solid Lipid Nanoparticles using 2% Apifil via ultrasonication effectively minimizes particle size and maximizes entrapment efficiency. Although it exhibits lower long-term storage stability at room temperature, it successfully preserves potent antioxidant activity, offering a promising approach for health sciences and advanced drug delivery applications
Ansel, Howard C, 2008. Pengantar Bentuk Sediaan Farmasi edisi IV. Jakarta: UI Press.
Avadi MR, Sadeghi AMM. Mohammad pour N, Abedin S, Atyabi F, Dinarvand R, Rafiee-Tehrani M. 2010. Preparation and characterization of insulin nanoparticles using chitosan and arabic gum with ionic gelation method. Nanomed Nanotechnol Biol Med, 6, 58-63. https://doi.org/10.1016/j.nano.2009.04.007
Boonme Prapaporn, Eliana B. Souto, Norasak Wuttisantikul, Tarntep Jongjit and Wiwat Pichayakorm. 2013. Influence of lipids on properties of solid lipid nanoparticles from microemulsion technique. European Journal of Lipid Science and Technology. https://doi.org/10.1002/ejlt.201200240
Chao Hong, Yang Danga, Guobei Lina, Yashu Yaoa, Guowen Li, Guang Ji, Hongyi Shen, Yan Xie. 2014. Effects of stabilizing agents on the development of myricetin nanosuspension and its characterization: An in vitro and in vivo evaluation. International Journal of Pharmaceutics. 251-260. https://doi.org/10.1016/j.ijpharm.2014.10.044
Cristina Prieto, Lourdes Calvo 2013 .Performance of the Biocompatible Surfactant Tween 80, for the Formation of Microemulsions Suitable for New Pharmaceutical Processing. Journal of Applied Chemistry. https://doi.org/10.1155/2013/930356
Jenning Volkhard, Sven Gohla. 2000. Comparison of wax and glyceride solid lipid nanoparticles. Jerman: Department of Pharmaceutics, Biopharmaceutics and Biotechnology. https://doi.org/10.1016/s0378-5173(99)00426-3
Landi-Librandi AP, de Oliveira CA,Azzolini AE, Kabeya LM, Del Ciampo JO, Bentley MV, Lucisano-Valim YM. 2011. In vitro evaluation of the antioxidant activity of liposomal flavonols by the HRP-H2O2-luminol system. J Microencapsul 28: 258–267 https://doi.org/10.3109/02652048.2011.559283
Nakahira, S. Nakamura, and M. Horimoto. 2007. Synthesis of Modified Hydroxyapatite (HAP) Substituted With Fe Ion for DDS Application. Japan. https://doi.org/10.1109/tmag.2007.894358
Qian J, Liang H, Somesfalean G, Wang D, He S, Zhang Z, Andersson-Engels S . 2011. Using 915 nm laser excited Tm³+/Er³+/Ho³+- doped NaYbF4 upconversion nanoparticles for in vitro and deeper in vivo bioimaging without overheating irradiation Bonifácio Bruna Vidal, Patricia Bento da Silva, Matheus Aparecido dos Santos Ramos, Kamil Maria Silveira Negri, Taís Maria Bauab, Marlus Chorilli. 2014. Nanotechnology-based drug delivery systems and herbal medicines: a review, International Journal of Nanomedicine, Brazil. https://doi.org/10.1021/nn200110j
Rawat, M., D. Singh, S. Saraf. 2006. Nanocarriers: Promising Vehicle for Bioactive Drugs. Biol. Pharm. Bull. 29(9) 1790-1798 . https://doi.org/10.1248/bpb.29.1790
Rosli, N.A. Hasham, R. Aziz, A. Aziz, R. 2015. Formulation and characterization of nanostructured lipid carrier encapsulated Zingiber zerumbet oil using ultrasonication technique. Journal of Advanced Research in Applied Mechanics. 11(1), 16-23. https://doi.org/10.11113/jt.v80.11268
Uner M, Yener G. 2007. Importance of solid lipid nanoparticles (SLN) in various administration routes and future perspectives. Int J Nanomedicine. 2(3):289-300. https://doi.org/10.1201/9780429111570-9
Xue M, Zhao Y, Li X-J, Jiang Z-Z, Zhang L, Liu S-H. 2012. Comparison of toxicokinetic and tissue distribution of triptolide-loaded solid lipid nanoparticles vs free triptolide in rats. Eur J Pharm Sci.47(4):713–7. https://doi.org/10.1016/j.ejps.2012.05.012
Zheng, Z., Zhang, Q., & Wu, J. 2010. DPPH-scavenging activities and structure-activity relationships of phenolic compounds. Natural Product Communications, 5(11), 1759–1765 https://doi.org/10.1177/1934578x1000501112
